Photochemical Regulation of Restriction Endonuclease Activity
نویسندگان
چکیده
منابع مشابه
Increasing cleavage specificity and activity of restriction endonuclease KpnI
Restriction enzyme KpnI is a HNH superfamily endonuclease requiring divalent metal ions for DNA cleavage but not for binding. The active site of KpnI can accommodate metal ions of different atomic radii for DNA cleavage. Although Mg(2+) ion higher than 500 μM mediates promiscuous activity, Ca(2+) suppresses the promiscuity and induces high cleavage fidelity. Here, we report that a conservative ...
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ژورنال
عنوان ژورنال: ChemBioChem
سال: 2009
ISSN: 1439-4227,1439-7633
DOI: 10.1002/cbic.200900090